Marijuana Research Today is a free monthly online journal that collates and summarizes the latest research about Marijuana, including details on benefits, cancer, effects, uses, addiction. | ||||||||
|
Improved gas chromatography-negative ion chemical ionization tandem mass spectrometric method for determination of 11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid in hair using mechanical pulverization and bead-assisted liquid-liquid extraction.Kim JY, Cheong JC, Lee JI, In MK Drug Analysis Laboratory, Forensic Science Division, Supreme Prosecutors' Office, 706 Banporo, Seocho-gu, Seoul 137-730, Republic of Korea. A gas chromatography-negative ion chemical ionization tandem mass spectrometric (GC-NCI-MS/MS) method was developed and validated for the determination of 11-nor-Δ(9)-tetrahydrocannabinol-9-carboxylic acid (THC-COOH) in human hair. After decontamination, hair samples were weighed (25mg), mechanically pulverized with a bead mill, and incubated in 0.7mL of 1.0M sodium hydroxide at 95°C for 30min. Bead-assisted liquid-liquid extraction was performed with n-hexane:ethyl acetate (9:1, v/v), a method developed in our laboratory. The extract was evaporated to dryness, derivatized with pentafluoropropanol and pentafluoropropionic anhydride, and analyzed by GC-MS/MS in the negative ion chemical ionization mode using methane as the reagent gas. The linear ranges were 0.05-10.0pg/mg for THC-COOH with the coefficient of determination (r(2)=0.9976). The intra-day and inter-day precisions were within 1.7 and 13.8%, respectively. The intra-day and inter-day accuracies were -4.8 to 10.0% and -3.9 to 3.8%, respectively. The limit of detection and quantification were 0.015 and 0.05pg/mg, respectively. The recoveries were in the range of 79.4-87.1%. The results indicate that the proposed method is simple, rapid, accurate, and precise for determination of THC-COOH in hair. The method identified THC-COOH in hair specimens from suspected marijuana abusers. Published 14 March 2011 in Forensic Sci Int, 206(1): e99-e102.
© 2004-2011 Marijuana Research Today. All Rights Reserved. |
| ||||||
